jsmsJournal of Siberian Medical SciencesJournal of Siberian Medical Sciences2542-1174Federal state budgetary educational institution of higher education "Novosibirsk state medical university" of Ministry of Health of the Russian Federation (FSBEI HE NSMU MOH Russia)10.31549/2542-1174-2025-9-4-105-120jsms-1280Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHТетрациклин снижает повреждение гиппокампа и мозжечка в модели легкой черепно-мозговой травмы у крыс подросткового возраста: кандидат на перепрофилирование?Tetracycline attenuates hippocampal and cerebellar damage in a weight-drop model of mild traumatic brain injury in adolescent rats: A candidate for drug repurposing?https://orcid.org/0009-0002-4889-7817АбариботеФ. А.AbariboteF. A.

Абариботе Физибе Амброуз – бакалавр, младший научный сотрудник

штат Байэлса, о-в Вилберфорс

Fiezibe Ambrose Abaribote – Bachelor of Science, Research Assistant

Bayelsa State, Wilberforce Island

https://orcid.org/0009-0009-9368-108XНвагорФ. КNwagorF. Ch.

Нвагор Фейсфул Крис – бакалавр, младший научный сотрудник

штат Байэлса, о-в Вилберфорс

Faithful Chris Nwagor – Bachelor of Science, Research Assistant

Bayelsa State, Wilberforce Island

https://orcid.org/0009-0009-7212-3695ОньекеГ. О.OnyekeG. O.

Оньеке Грэйс Ойома – бакалавр, младший научный сотрудник

штат Байэлса, о-в Вилберфорс

Grace Ojoma Onyeke – Bachelor of Science, Research Assistant

Bayelsa State, Wilberforce Island

https://orcid.org/0000-0001-7911-1392ОйинбоЧ. А.OyinboCh. A.

Ойинбо Чарльз Айдемайз – PhD, доцент

штат Байэлса, о-в Вилберфорс

Charles Aidemise Oyinbo – PhD, Associate Professor

Bayelsa State, Wilberforce Island

charles.oyinbo@ndu.edu.ngУниверситет дельты Нигера, факультет базовой медицины, кафедра анатомии человекаНигерияNiger Delta University, Department of Human Anatomy, Faculty of Basic Medical SciencesNigeria20252612202504105120Copyright © Абариботе Ф.А., Нвагор Ф.К., Оньеке Г.О., Ойинбо Ч.А., 20252025Абариботе Ф.А., Нвагор Ф.К., Оньеке Г.О., Ойинбо Ч.А.Abaribote F.A., Nwagor F.C., Onyeke G.O., Oyinbo C.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://jsms.elpub.ru/jour/article/view/1280Введение

Введение . Легкая черепно-мозговая травма (ЛЧМТ) может стать критической для нормального развития подросткового организма вследствие поражения областей головного мозга, ответственных за когнитивные и двигательные функции. Современные методы лечения ЛЧМТ, основанные в большинстве своем на опыте лечения этого состояния у взрослых, слабо ориентированы на подростков, что вызывает необходимость разработки стратегий терапии для этой группы пациентов.

Цель

Цель . Данное исследование изучает потенциальные нейропротективные эффекты тетрациклина у крысподростков стока Вистар после ЛЧМТ, уделяя особое внимание повреждению гиппокампа и коры мозжечка.

 Материалы и методы

 Материалы и методы. Пятнадцать крыс были разделены на три группы: контрольную, группу с ЛЧМТ и группу с ЛЧМТ + тетрациклин в суточной дозе 44,3 мг/кг массы тела. ЛЧМТ была моделирована методом падающего груза, когнитивные и моторные функции оценивались в тестах Т-образного лабиринта и суживающейся дорожки.

Результаты

Результаты. Гистологический анализ с использованием окрашивания гематоксилином и эозином и оценка поведенческих реакций показали, что у крыс с ЛЧМТ без введения тетрациклина наблюдались выраженные негативные изменения в функционировании и структуре головного мозга, а также нарушение когнитивных и двигательных функций. В то же время у крыс, получавших тетрациклин, негативные изменения нейрональной структуры и поведения были менее выражены, хотя и полностью не восстанавливались до уровня контрольной группы. Применение тетрациклина также уменьшало воспаление и окислительный стресс в головном мозге.

Заключение

Заключение . Полученные результаты показывают, что тетрациклин обладает нейропротекторным потенциалом при ЛЧМТ у крыс-подростков, что обосновывает его изучение в качестве кандидата для перепрофилирования.

Introduction

Introduction. Mild traumatic brain injury (mTBI) represents a critical risk factor for disrupted neurodevelopment during adolescence, particularly affecting brain regions involved in cognition and motor function. Current treatments, which are mostly derived from adult studies, highlight a gap in addressing adolescent needs, warranting the need for targeted therapeutic strategies.

Aim

Aim. This study explores the potential neuroprotective effects of tetracycline in adolescent Wistar rats following mTBI, focusing on damage to the hippocampus and cerebellar cortex. mTBI poses a significant risk to brain development in adolescents.

 Materials and methods

 Materials and methods . Fifteen rats were divided into three groups: a control group, an injury group only, and an injury group treated with tetracycline at a daily dose of 44,3 mg/kg body weight. mTBI was induced using a weight-drop method, and cognitive and motor functions were assessed through T-maze and beam-walking tests.

Results

Results . Histological analysis using hematoxylin and eosin staining revealed that untreated rats with mTBI showed significant neural degeneration and disrupted brain architecture, alongside impaired cognitive and motor performance. In contrast, rats treated with tetracycline exhibited reduced neuronal damage and improved performance, although not fully restored to control levels. The treatment also appeared to lower inflammation and oxidative stress in the brain.

Conclusion

Conclusion. These findings suggest that tetracycline has neuroprotective potential in adolescent rats with mTBI, supporting its consideration as a therapeutic target for drug repurposing.

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The authors declare that there are no conflicts of interest present.