jsmsJournal of Siberian Medical SciencesJournal of Siberian Medical Sciences2542-1174Federal state budgetary educational institution of higher education "Novosibirsk state medical university" of Ministry of Health of the Russian Federation (FSBEI HE NSMU MOH Russia)jsms-242Research Article14.00.00 МЕДИЦИНСКИЕ НАУКИ (14.03.00 МЕДИКО-БИОЛОГИЧЕСКИЕ НАУКИ)ДЕКСТРАН ПОВЫШАЕТ СООТНОШЕНИЕ ИФН-Γ/ИЛ-10 В МАКРОФАГАХ ЧЕЛОВЕКА, ЗАРАЖЕННЫХ МИКОБАКТЕРИЯМИ ТУБЕРКУЛЕЗАDEXTRAN RAISES RATIO IFN-Γ / IL-10 IN MACROPHAGES OF THE PERSON INFECTED WITH TUBERCULOSIS MYCOBACTERIAПустыльниковС. В.PustylnikovS. V.sergey.pustylnikov@gmail.comФГБУ «Новосибирский научно-исследовательский институт туберкулёза» Минздрава РоссииРоссияFBHE «Novosibirsk SRI of tuberculosis» of Ministry of HealthRussian Federation201504062018043636Copyright © Пустыльников С.В., 20182018Пустыльников С.В.Pustylnikov S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://jsms.elpub.ru/jour/article/view/242

Целью работы было изучение - каким образом декстран в качестве лиганда маннозного рецептора влияет на цитокиновый ответ макрофагов человека при заражении микобактериями. Макрофаги инкубировали с декстраном, а затем заражали микобактериями туберкулеза H37Rv. Через 1 и 7 дней надклеточную жидкость собирали и анализировали концентрации ИФН-γ и ИЛ-10. Декстран повысил соотношение ИФН-γ/ИЛ-10 в 2,4 раза. Это свидетельствует о сдвиге активации макрофагов в сторону классического пути (М1), что способствует формированию Th1 иммунного ответа, необходимого для благоприятного исхода при туберкулезе.

The purpose of the research was studying of dextran as ligand of mannose receptor influences on cytokine response of macrophages of the person at infection with micobacteria. Macrophages were incubated with dextran, and then they were infected with H37Rv tuberculosis micobacteria. In 1 and 7 days supracellular liquid was collected and analyzed on concentration of IFN-γ and IL-10. The dextran raised ratio of IFN-γ/IL-10 by 2,4 times. It testifies to shift of activation of macrophages towards a classical way (M1) that promotes formation of Th1 of the immune response that is necessary for a benign outcome at tuberculosis.

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The authors declare that there are no conflicts of interest present.