jsmsJournal of Siberian Medical SciencesJournal of Siberian Medical Sciences2542-1174Federal state budgetary educational institution of higher education "Novosibirsk state medical university" of Ministry of Health of the Russian Federation (FSBEI HE NSMU MOH Russia)jsms-453Research ArticleСтатьиEstimation of connexin 43 in myocard by experimental modeling of acute ischemiaSavchenkoS. V.dr.serg62@yandex.ruNovoselovV. P.noemail@neicon.ruSkrebovR. V.noemail@neicon.ruMorozovaA. S.noemail@neicon.ruGritsingerV. A.noemail@neicon.ruAgeevaT. A.noemail@neicon.ruAidagulovaS. V.noemail@neicon.ruErschovK. I.noemail@neicon.ruVoroninaE. I.noemail@neicon.ruNovosibirsk State Medical UniversityRussian Federation201704062018011923Copyright © Savchenko S.V., Novoselov V.P., Skrebov R.V., Morozova A.S., Gritsinger V.A., Ageeva T.A., Aidagulova S.V., Erschov K.I., Voronina E.I., 20182018Savchenko S.V., Novoselov V.P., Skrebov R.V., Morozova A.S., Gritsinger V.A., Ageeva T.A., Aidagulova S.V., Erschov K.I., Voronina E.I.Savchenko S.V., Novoselov V.P., Skrebov R.V., Morozova A.S., Gritsinger V.A., Ageeva T.A., Aidagulova S.V., Erschov K.I., Voronina E.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://jsms.elpub.ru/jour/article/view/453

We induced acute ischemia by ligation of the left coronary artery in the area between left atrial appendage and pulmonary trunk. Animals were sacrificed after 1 hour, 3 hours, 12 and 24 hours. Using immunohistochemical methods to determine the intensity of connexin 43 in cardiomyocytes allows to identify sites of acute ischemic myocardial damage. We estimated the distribution of connexin in the area of intercalated discs and Zbands in the myocardium by experimental modeling of acute ischemia in laboratory male rats of “Wistar” line. Immunohistochemical research of myocardium carried out with the purpose to estimate expression severity of connexin 43 can be recommended for use in cases of morphological examination of dead after sudden cardiac death for diagnostic of myocardial ischemia.

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The authors declare that there are no conflicts of interest present.