jsmsJournal of Siberian Medical SciencesJournal of Siberian Medical Sciences2542-1174Federal state budgetary educational institution of higher education "Novosibirsk state medical university" of Ministry of Health of the Russian Federation (FSBEI HE NSMU MOH Russia)10.31549/2542-1174-2019-1-4-17jsms-552Research ArticleСтатьиСвязь изменений относительной длины теломер в течение пятнадцати лет с маркерами возрастных изменений сердечно-сосудистой системы у лиц среднего возраста по результатам исследования питания и рака в Мальмё (Швеция)Change in relative telomere length over fifteen years is associated with markers of cardiovascular ageing in middle-aged subjects – the Malmö Diet and Cancer Study, SwedenНильсонП. М.NilssonP. M.

Нильсон Питер М. — MD, PhD, профессор (сердечно-сосудистые болезни). Отделение клинических наук, Исследовательская группа по сердечно-сосудистой генетике и сердечно-сосудистым болезням.

Jan Waldenstroms gata, 15, 5-й этаж, университетская больница Сконе, S-205 02, Мальмё.

Nilsson Peter M. — MD, PhD, Professor of Clinical Cardiovascular Research. Department of Clinical Sciences, Cardiovascular Genetics and Clinical Cardiovascular Research Group.

Jan Waldenstroms gata, 15, 5th floor, Skåne University Hospital, S-205 02, Malmö.

Peter.Nilsson@med.lu.seДальманД.DahlmanD.

Дальман Диса — MD, PhD, врач. Отделение клинических наук, Исследовательская группа по сердечнососудистой генетике и сердечно-сосудистым болезням.

Jan Waldenstroms gata, 15, 5-й этаж, университетская больница Сконе, S-205 02, Мальмё.

Dahlman Disa — MD, PhD, Registrar Phisician. Department of Clinical Sciences, Cardiovascular Genetics and Clinical Cardiovascular Research Group.

Jan Waldenstroms gata, 15, 5th floor, Skåne University Hospital, S-205 02, Malmö.

МеландерО.MelanderO.

Меландер  Олле  —  MD,  PhD,  профессор  (внутренние болезни). Отделение клинических наук, Исследовательская группа по сердечно-сосудистой генетике и сердечно-сосудистым болезням.

Jan Waldenstroms gata, 15, 5-й этаж, университетская больница Сконе, S-205 02, Мальмё.

Melander Olle — MD, PhD, Professor of Internal Medicine. Department of Clinical Sciences, Cardiovascular Genetics and Clinical Cardiovascular Research Group.

Jan Waldenstroms gata, 15, 5th floor, Skåne University Hospital, S-205 02, Malmö.

Университет Лунда, Университетская больница СконеШвецияLund University, Skåne University HospitalSweden20192407201901417Copyright © Нильсон П.М., Дальман Д., Меландер О., 20192019Нильсон П.М., Дальман Д., Меландер О.Nilsson P.M., Dahlman D., Melander O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://jsms.elpub.ru/jour/article/view/552Цель

Цель . Проанализировать связь между длительными изменениями относительной длины теломер (ОДТ) и отдельными маркерами возрастных изменений сердечно-сосудистой системы, такими как инфаркт миокарда (ИМ) в анамнезе, пульсовое давление или прием антигипертензивных препаратов (АГП) за 15-летний период наблюдения.

Материалы  и  методы

Материалы  и  методы .  В исследовании приняли участие 332 пациента среднего возраста с ИМ в анамнезе. ОДТ крови при первичном обследовании и спустя 15 лет определялась методом количественной ПЦР. Для сравнения логарифмического распределения ОДТ на начальном и финальном этапах исследования использовался z-тест. Разница (Δz-оценка) рассчитывалась для каждого больного как маркер относительного изменения длины теломер за 15 лет. Множественный регрессионный анализ проводился с Δz-оценкой как зависимой переменной; независимыми переменными являлись возраст, пол, исходный уровень ОДТ и клинические маркеры возрастных изменений сердечно-сосудистой системы.

Результаты

Результаты .  Установлено, что исходно Δz-оценка ОДТ независимо связана как со случаями (дебютными/ повторными) ИМ (p < 0.039), так и с приемом АГП (p < 0.015) на момент начала исследования. У лиц с ИМ в анамнезе или принимающих АГП наблюдалось большее снижение z-оценки для ОДТ за период исследования, что свидетельствует о более высокой степени укорочения теломер.

Заключение

Заключение . Изменения ОДТ (их истощение) крови являются потенциальным маркером возрастных изменений сердечно-сосудистой системы, что подтверждается наличием значимой и независимой корреляционной связи между Δz-оценкой и дебютным/повторным ИМ, а также исходным приемом АГП, являющимися признаками многолетней гипертонии, требующей лечения.

Objective

Objective.  To analyse the association between long-term changes in relative telomere length (RTL) and selected markers of cardiovascular ageing such as history of myocardial infarction (MI), pulse pressure or usage of antihypertensive (AHT) drugs during 15 years of follow-up.

Subjects  and  methods

Subjects  and  methods.  332 middle-aged subjects were recruited from a population-based, case-control study of patients with MI. RTL in blood at baseline and after 15 years of follow-up was investigated through quantitative PCR. Z-statistics was used to compare the log-distribution of RTL at baseline and at follow-up, respectively. The difference (Δz-score) was calculated for each individual as a marker of relative change in telomere length over 15 years. Multiple regression analysis was used with Δz-score as dependent variable, and age, sex, RTL at baseline, and clinical markers of cardiovascular ageing as independent variables.

Results

Results.  We found that Δz-score of RTL was independently associated with both total (prevalent/incident) MI (p < 0.039) and usage of AHT drugs (p < 0.015) at baseline. Subjects with MI or on AHT drugs showed a greater reduction in the z-score for RTL during follow-up, implying a higher degree of telomere shortening.

Conclusion

Conclusion.  Changes in blood relative telomere attrition (RTL) is a potential marker of cardiovascular ageing, as illustrated by significant and independent associations between Δ z-score and prevalent/incident MI, as well as with base-line usage of antihypertensive drugs — a marker of longstanding hypertension in need of treatment.

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The authors declare that there are no conflicts of interest present.