jsmsJournal of Siberian Medical SciencesJournal of Siberian Medical Sciences2542-1174Federal state budgetary educational institution of higher education "Novosibirsk state medical university" of Ministry of Health of the Russian Federation (FSBEI HE NSMU MOH Russia)10.31549/2542-1174-2021-1-4-11jsms-679Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESМикробиота плодных оболочек при интактном плодном пузыре и доношенной беременностиMicrobiota of fetal membranes in intact amniotic sac and full-term pregnancyКагановаМ. А.KaganovaM. A.

Каганова Мария Александровна — кандидат медицинских наук, доцент кафедры акушерства и гинекологии.

443079, Самара, ул. Чапаевская, 89

Kaganova Mariya Aleksandrovna — Cand. Sci. (Med.), Assistant Professor, Obstetrics and Gynecology Department.

89, Chapayevskaya str., Samara, 443079

mkaganova@yandex.ruСпиридоноваН. В.SpiridonovaN. V.

Спиридонова Наталья Владимировна — доктор медицинских наукнаук, профессор, заведующий кафедрой акушерства и гинекологии.

Самара

Spiridonova Natalya Vladimirovna — Dr. Sci. (Med.), Professor, Head, Obstetrics and Gynecology Department.

Samara

МахлинаЕ. А.MakhlinaE. A.

Махлина Елена Алексеевна — врач акушер-гинеколог.

Самара

Makhlina Elena Alekseevna — Obstetrician-gynecologist.

Samara

ФГБОУВО Самарский государственный медицинский университет Минздрава РоссииРоссияSamara State Medical UniversityRussian FederationКонсультативная клиника Панацея, ОООРоссияConsultative clinic Panatseya, LLCRussian Federation20210904202101411Copyright © Каганова М.А., Спиридонова Н.В., Махлина Е.А., 20212021Каганова М.А., Спиридонова Н.В., Махлина Е.А.Kaganova M.A., Spiridonova N.V., Makhlina E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://jsms.elpub.ru/jour/article/view/679Цель исследования

Цель исследования. Изучить микробный пейзаж плодных оболочек при интактном плодном пузыре и доношенной беременности

Материалы и методы

Материалы и методы. У 19 беременных (средний возраст 31.0 ± 5.3 года, средний срок гестации 39.3 ± 0.65 нед) с неповрежденными плодными оболочками во время элективного кесарева сечения проводился забор ткани плодных оболочек с целью выявления следующих микроорганизмов методом полимеразной цепной реакции: Lactobacillus spp., Enterobacteriaceae, Streptococcus spp., Staphylococcus spp., Gardnerella vaginalis/Pre-votella bivia/Porphyromonas spp., Eubacterium spp., Sneathia spp./Leptotrihia spp./ Fusobacterium spp., Megasphaera spp./Veillonella spp./ Dialister spp., Lachnobacterium spp./Clostridium spp., Mobiluncus spp./Corynebacterium spp., Peptostreptococcus spp., Atopobium vaginae, Mycoplasma hominis, Ureaplasma (urealyticum + parvum), Candida spp., Mycoplasma genitalium.

Результаты

Результаты. Стерильные оболочки обнаружены у 5 беременных (26.3 %), в остальных случаях выявлена общая бактериальная масса (ОБМ) 1045 (1035-105-8) геном-эквивалентов (ГЭ)/образец. Преобладали представители семейства Enterobacteriaceae — в среднем 1045 ГЭ/образец, лишь в одном случае выявлена Candida spp. В 42.1 % случаев при выявлении ОБМ конкретные виды микроорганизмов идентифицированы не были.

Заключение

Заключение. На плодных оболочках при доношенной беременности допустимо присутствие ОБМ в среднем 1045 (1035-105-8) ГЭ/образец, в которой преобладают Enterobacteriaceae в среднем 1045 ГЭ/образец.

Aim of research

Aim of research. To study the microbial landscape of intact fetal membranes in full-term pregnancy.

Materials and methods

Materials and methods. In 19 pregnant women (mean age — 31.0 ± 5.3 years, mean gestational age — 39.3 ± 0.65 weeks) with intact fetal membranes, the fetal membrane tissue was collected during elective cesarean section to detect by polymerase chain reaction the following microorganisms: Lactobacillus spp., Enterobacteriaceae, Streptococcus spp., Staphylococcus spp., Gardnerella vaginalis/Prevotella bivia/Porphyromonas spp., Eubacterium spp., Sneathia spp./Leptotrihia spp./ Fusobacterium spp., Megasphaera spp./Veillonella spp./ Dialister spp., Lachnobacterium spp./Clostridium spp., Mobiluncus spp./Corynebacterium spp., Peptostreptococcus spp., Atopobium vaginae, Mycoplasma hominis, Ureaplasma (urealyticum + parvum), Candida spp., Mycoplasma genitalium.

Results

Results. Sterile membranes were found in 5 pregnant women (26.3%), in the remaining cases, the total bacterial load (TBL) was 1045 (1035-1058) genome equivalents (GE) per sample. Representatives of the Enterobacteriaceae family prevailed — 1045 GE per sample on average, only in one case Candida spp. were detected. In 42.1% of cases, when determining TBL, specific types of microorganisms were not identified.

Conclusion

Conclusion. On the fetal membranes in full-term pregnancy, the average TBL corresponding to 1045 (1035105 8) GE per sample, in which Enterobacteriaceae prevail in the amount of 104 5 GE per sample on average, is acceptable.

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The authors declare that there are no conflicts of interest present.