jsmsJournal of Siberian Medical SciencesJournal of Siberian Medical Sciences2542-1174Federal state budgetary educational institution of higher education "Novosibirsk state medical university" of Ministry of Health of the Russian Federation (FSBEI HE NSMU MOH Russia)10.31549/2542-1174-2022-6-1-22-37jsms-790Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHПрофиль экспрессии ряда микроРНК в плаценте при своевременных и преждевременных родахThe expression profile of a number of miRNAs in the placenta in timely and premature birthhttps://orcid.org/0000-0002-4512-3421КазачковЕ. Л.KazachkovE. L.

 д-р мед. наук, профессор, заведующий кафедрой патологической анатомии и судебной медицины им. проф. В.Л. Коваленко 

 454092, г. Челябинск, ул. Воровского, 64

 Dr. Sci. (Med.), Professor, Head, Department of Pathological Anatomy and Forensic Medicine named after prof. V.L. Kovalenko

64, Vorovsky str., Chelyabinsk, 454092, Russia 

doctorkel@yandex.ruhttps://orcid.org/0000-0002-3268-7981СемёновЮ. А.SemenovYu. A.

 канд. мед. наук, доцент кафедры акушерства и гинекологии 

 454092, г. Челябинск, ул. Воровского, 64

 Cand. Sci. (Med.), Associate Professor, Department of Obstetrics and Gynecology 

64, Vorovsky str., Chelyabinsk, 454092, Russia 

https://orcid.org/0000-0002-3799-9407ВеряскинаЮ. А.VeryaskinaYu. A.

канд. биол. наук, научный сотрудник лаборатории молекулярной генетики 

Новосибирск 

 Cand. Sci. (Biol.), Researcher, Laboratory of Molecular Genetics

 Novosibirsk

https://orcid.org/0000-0002-1672-7058КазачковаЭ. А.KazachkovaE. A.

 д-р мед. наук, профессор кафедры акушерства и гинекологии 

 454092, г. Челябинск, ул. Воровского, 64

 Dr. Sci. (Med.), Professor, Department of Obstetrics and Gynecology 

64, Vorovsky str., Chelyabinsk, 454092, Russia 

https://orcid.org/0000-0003-1574-1613ЧижовскаяА. В.ChizhovskayaA. V.

аспирант кафедры патологической анатомии и судебной медицины им. проф. В.Л. Коваленко 

 454092, г. Челябинск, ул. Воровского, 64

 Graduate, Department of Pathological Anatomy and Forensic Medicine named after prof. V.L. Kovalenko 

64, Vorovsky str., Chelyabinsk, 454092, Russia 

ФГБОУ ВО «Южно-Уральский государственный медицинский университет» Минздрава РоссииРоссияSouth Ural State Medical University Russian FederationФГБУН Институт молекулярной и клеточной биологии СО РАНРоссияInstitute of Molecular and Cellular Biology Russian Federation202228032022012237Copyright © Казачков Е.Л., Семёнов Ю.А., Веряскина Ю.А., Казачкова Э.А., Чижовская А.В., 20222022Казачков Е.Л., Семёнов Ю.А., Веряскина Ю.А., Казачкова Э.А., Чижовская А.В.Kazachkov E.L., Semenov Y.A., Veryaskina Y.A., Kazachkova E.A., Chizhovskaya A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://jsms.elpub.ru/jour/article/view/790

Введение. Сравнительный анализ уровней экспрессии ряда микроРНК (миРНК) в плацентах, полученных при своевременных и спонтанных преждевременных родах (ПР), позволит выявить те миРНК, которые участвуют в генезе спонтанных ПР. МиРНК, характеризующиеся аберрантной экспрессией в тканях плаценты, могут являться перспективными биомаркерами в крови беременных для оценки риска спонтанных ПР.Цель. Сравнительный анализ уровней экспрессии ряда микроРНК в ткани плаценты у женщин при своевременных и преждевременных родах.Материалы и методы. Проведен анализ уровня экспрессии ряда миРНК в ткани плаценты от 30 пациенток с ПР и перинатальными потерями (1-я группа), от 30 пациенток с ПР без перинатальных потерь (2-я группа), от 30 родильниц со своевременными родами (3-я группа). Изучали уровень экспрессии следующих миРНК: 31, 100, 146, 150, 20a, 204, 221, 223, 1246, 128, let7a, 126, 451, 92a, 23a, 21, 125b, 26a, 29b, 191 и U6. Для этого поэтапно проводили депарафинизацию материала с использованием минерального масла, экстракцию РНК, реакцию обратной транскрипции и полимеразную цепную реакцию в реальном времени.Результаты.. Установлено, что при ПР в образцах плацент отмечается статистически значимое увеличение уровней экспрессии миРНК-125b, миРНК-29b и снижение уровня экспрессии миРНК-451 по сравнению с плацентами при своевременных родах. При наличии ПР с перинатальными потерями зарегистрировано статистически значимое снижение уровня экспрессии миРНК-150 в плаценте, а при отсутствии перинатальных потерь – увеличение уровня экспрессии миРНК-223 и миРНК-31 по сравнению с плацентами при своевременных родах. Кроме того, при ПР с перинатальными потерями по сравнению с ПР без перинатальных потерь отмечается статистически значимо более низкий уровень экспрессии миРНК-221 и миРНК-223.Заключение.. Аберрантные значения уровней экспрессии миРНК-125b, миРНК-29b и миРНК-451 в плацентах пациенток с ПР свидетельствуют об их участии в патогенезе последних, по-видимому, за счет нарушения регуляции процессов ангиогенеза, апоптоза, инвазии трофобласта и метаболизма глюкозы.

Introduction.  Comparative analysis of the expression levels of a number of microRNAs (miRNAs) in placentas obtained during timely and spontaneous preterm birth (PB) will make it possible to identify those miRNAs that are involved in the genesis of spontaneous PB. MiRNAs characterized by aberrant expression in placental tissues may be promising biomarkers in the blood of pregnant women for assessing the risk of spontaneous PB.Aim. Comparative analysis of expression levels of a number of microRNAs in placental tissue in women in timely and premature birth.Materials and Methods. We performed an analysis of the expression level of a number of miRNAs in placental tissue from 30 patients with PB and perinatal losses (group 1), from 30 patients with PB without perinatal losses (group 2), from 30 maternity women with timely delivery (group 3). The expression levels of the following miRNAs were studied: 31, 100, 146, 150, 20a, 204, 221, 223, 1246, 128, let7a, 126, 451, 92a, 23a, 21, 125b, 26a, 29b, 191, and U6. For this, the material was deparaffinated step by step using mineral oil, then the RNA extraction, reverse transcription reaction, and real-time polymerase chain reaction were carried out.Results. It was found that in PB a statistically significant increase in the expression levels of miRNA-125b, miRNA-29b is observed in placental samples, and a decrease in the expression level of miRNA-451 in comparison with timely delivery placentas. In the PB with perinatal losses, a statistically significant decrease in the expression level of miRNA-150 was registered, and in the absence of perinatal losses, an increase in the expression level of miRNA-223 and miRNA-31 compared with placentas in timely delivery was revealed. In addition, in PB with perinatal losses compared to PB without perinatal losses, a statistically significantly lower level of expression of miRNA-221 and miRNA-223 is noted.Conclusion Aberrant expression levels of miRNA-125b, miRNA-29b, and miRNA-451 in the placentas of patients with PB indicate their involvement in the pathogenesis of the latter, apparently due to dysregulation of angiogenesis, apoptosis, trophoblast invasion and glucose metabolism.

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The authors declare that there are no conflicts of interest present.