An approach to standardization of Nonea rossica Steven herb

Abstract

I n t r o d u c t i o n. The reproducibility of the effect of any herbal medicinal product or its effectiveness depends on the quantitative variability of the chemical composition of medicinal plant raw materials (MPRM).

A i m. A study of the individual variability of biologically active compounds (BAC) of the phenolic complex of Nonea (N.  rossica Steven) herb and the determination of statistically valid quality criteria for MPRM.

M a t e r i a l s  a n d  m e t h o d s . The objects of the study were specimens of N. rossica Steven herb obtained from plants collected at different points of the range in its typical habitats during the flowering phase in 2017–2022. The content of BAC markers – flavonoids, cinnamic acids and lactone compounds was determined by spectrophotometry.

R e s u l t s. As a result of the analysis of the content of BAC markers of 12 plants growing in one cropping area, it was found that the coefficient of variation does not exceed 24%, so, the studied sample is uniformed. The homogeneity of the sample makes it possible to statistically correctly combine individual specimens and form a combined sample for plants collected within the cropping area. An analysis of the content of BAC markers in raw materials collected at various points of the range (combined samples) showed that the coefficient of variation increased slightly and did not exceed the critical value of 33%. Here, the combined samples under consideration are also homogeneous, which makes it possible to determine confidence intervals for varying the content of BAC markers.

C o n c l u s i o n. The criteria for the quality of raw materials have been established: the content of flavonoids in terms of rutin should be at least 0.68%, cinnamic acids in terms of caffeic acid – at least 1.9%, lactones in terms of 2-hydroxycinnamicacid – at least 0.25%.