Development of a method for quantitative determination of the hydrophilic antioxidant TF-7 substance by stri pping voltammetry

Abstract

Introduction. Hydrophilic antioxidants are quite attractive for use in biology, veterinary practice and medicine because of their high bioavailability and transport rates, convenience of administration and dosing, suitable for use in infusion therapy in acute conditions. Traditional use of phenolic antioxidants did not presuppose their hydrophilic properties; rather, lipophilicity was preferred. In this regard, it is not surprising that the number of known water-soluble forms of phenolic antioxidants is very limited.

Aim. Quantitative determination of the water-soluble antioxidant TF-7 substance by stripping voltam metry. Materials and methods. An object of the study was a new pharmaceutical substance of the water-soluble antioxidant TF-7 (3-(4-hydroxy-3,5-dimethylbenzylthio) potassium propionate). For quantitative determination, stripping voltammetry was used.

Results. The main parameters of the method, in particular sweep rate, time and potential of accumulation, which have the greatest infl uence on the quantitative characteristics of the analytical signal, were determined. The dependence of amount of the substance on concentration and the minimum detectable concentration are established. Optimal background electrolytes were selected, on which the analytical signal appears. Assessment of linearity showed the coeffi cient of linearity for the method developed was 0.997.

Conclusion. The selected parameters (background electrolyte, working electrode and reference electrode, accumulation potential and time, start and rate of sweep, mixing time) make it possible to quantify the water-soluble antioxidant TF-7 substance.