Functional state of erythrocytes under the extreme physical exertion while supplemented fish oil and ozonated fish oil

In t r o d u c t i o n . The extreme physical exertion in athletes is a powerful stress factor for the organism and causes an increase in the level of stress hormones, vasoconstriction thus resulting in the microcirculation system disorders, which are largely infl uenced by erythrocytes. In turn, the movement of erythrocytes through the microcirculatory bed is determined by the state of the physicochemical properties of their membrane depending on the type of lipid phase fatty acids. At the same time, it has been shown that professional athletes have a defi ciency of omega-3 polyunsaturated fatty acids (n-3 PUFAs). The content of n-3 PUFAs in fi sh oil (FO) determines its use in practice of sports medicine. At the same time, the eff ectiveness of FO and its ozonated form on the state and functioning of erythrocytes under conditions of temporary homeostasis imbalance due to the extreme physical exertion remains unclear.

A i m . Study of the eff ect of FO and ozonated FO (OFO) on the functional indices of erythrocytes in rats under the extreme physical exertion.

Ma t e r i a l s a n d m e t h o d s . The eff ect of FO and OFO on metabolic and oxidative indices of erythrocytes, erythrocyte electrophoretic mobility (EEM) and their aggregation during modeling of physical activity in rats was studied. Extreme physical exertion in animals was modeled with forced swimming with a load equal to 10% of their body weight. The weight-loaded forced swimming (stress) test was performed 4 times; during the post-exertion recovery, the rats received FO, OFO (with an ozonide number of 3000 or 1500 – OFO-3000 and OFO-1500, respectively) depending on the group. The animals were tested 6 times: parameters were determined at baseline, after the stress test (4 times) and after substance withdrawal (on the 3rd day). The concentration of malondialdehyde (MDA), adenosine triphosphate (ATP), 2,3-diphosphoglycerate (DPG), EEM and erythrocyte aggregation was measured.

R e s u l t s . The extreme physical exertion (control group) caused an increase in the MDA concentration, a decrease in EEM and the concentration of ATP and 2,3-DPG in erythrocytes, which was accompanied by an increase in erythrocyte aggregation. The administration of FO during physical exertion determined less pronounced lipid peroxidation, while the administration of OFO-3000 determined induced the most pronounced lipid peroxidation compared to the control group. The lowest oxidative eff ect during physical exertion was recorded with the administration of OFO-1500. A decrease in lipid peroxidation was accompanied by an increase in the ATP concentration, a decrease in 2,3-DPG, an increase in EEM and a decrease in erythrocyte aggregation.

Co n c l u s i o n . The use of OFO-1500 most eff ectively eliminated the processes in rat erythrocytes induced by stress caused by extreme physical exertion.

1. Armstrong M.E.G., Green J., Reeves G.K. et al. Frequent physical activity may not reduce vascular disease risk as much as moderate: Large prospective study of women in the United Kingdom. Circulation. 2015;131(8):721729. DOI: 10.1161/CIRCULATIONAHA.114.010296.

2. Gostyukhina A.A., Zamoshchina T.A., Svetlik M.V. et al. Behavioral activity of rats in the «open fi eld» after the light and dark deprivation and physical exhaustion. Bulletin of Siberian Medicine. 2016;15(3):16-23. DOI: 10.20538/1682-0363-2016-3-16-23. (In Russ.)

3. Grigorjeva M.E., Sorokoletov S.M., Korobovsky A.V., Lyapina L.A. Blood fl uidity during physical exertion of various types. Sports medicine: research and practice. 2022;12(4):45-58. DOI: 10.47529/22232524.2022.4.3. (In Russ.)

4. Medeiros-Lima D.J., Mendes-Ribeiro A.C., Brunini T.M. et al. Erythrocyte nitric oxide availability and oxidative stress following exercise. Clin. Hemorheol. Microcirc. 2017;65(3):219-228. DOI: 10.3233/CH-16162.

5. Kushnerova N.F., Rakhmanin Yu.A., Momot T.V. et al. Assessment of changes in the lipid composition of blood plasma and erythrocyte membranes in students under study load and their prevention. Hygiene and Sanitation, Russian journal. 2020;99(2):187-192. DOI: 10.33029/0016-9900-2020-99-2-187-192. (In Russ.)

6. Kruglova I.V., Davidyan O.V. Eff ectiveness of omega-3 polyunsaturated fatty acids in a complex of recovery measures for athletes. Vopr. Dietol. (Nutrition). 2017;7(3):20-27. DOI: 10.20953/2224-5448-2017-3-20-27. (In Russ.)

7. Siener R., Alteheld B., Terjung B. et al. Change in the fatty acid pattern of erythrocyte membrane phospholipids after oral supplementation of specifi c fatty acids in patients with gastrointestinal diseases. Eur. J. Clin. Nutr. 2010;64(4):410-418. DOI: 10.1038/ejcn.2009.151.

8. Zaloga G.P. Narrative review of n-3 polyunsaturated fatty acid supplementation upon immune functions, resolution molecules and lipid peroxidation. Nutrients. 2021;13(2):662. DOI: 10.3390/nu13020662.

9. Joumard-Cubizolles L., Lee J.C., Vigor C. et al. Insight into the contribution of isoprostanoids to the health eff ects of omega 3 PUFAs. Prostaglandins Other Lipid Mediat. 2017;133:111-122. DOI: 10.1016/j.prostaglandins.2017.05.005.

10. Stupin M., Kibel A., Stupin A. et al. The physiological eff ect of n-3 polyunsaturated fatty acids (n-3 PUFAs) intake and exercise on hemorheology, microvascular function, and physical performance in health and cardiovascular diseases; is there an interaction of exercise and dietary n-3 PUFAs intake? Front. Physiol. 2019;10:1129. DOI: 10.3389/fphys.2019.01129.

11. Criegee R. Mechanism of ozonolysis. Angew. Chem. Int. Ed. Engl. 1975;14(11):745-752.

12. Peretyagin S.P., Gordetsov A.S., Soloveva A.G. et al. The infl uence of low-frequency electropulse exposion on the physicochemical parameters and biological activity of a cream containing reactive oxygen species. Bioradicals and Antioxidants. 2017;4(4):57-64. (In Russ.)

13. Deryugina A.V., Oshevenskiy L.V., Talamanova M.N. et al. Changes in electrokinetic and biochemical characteristics in erythrocytes under the action of terahertz electromagnetic waves. Biophysics. 2017;62(6):11081113. (In Russ.)

14. Deryugina A.V., Gracheva E.A. Efficacy of cytoflavin administration in rats with experimental arterial hypertension. Experimental and Clinical Pharmacology. 2020;83(2):8-11. DOI: 10.30906/0869-2092-2020-83-2-8-11. (In Russ.)

15. Vinogradova I.L., Bagryantseva S.Yu., Derviz G.V. Method for the simultaneous determination of 2,3-DPG and ATP in erythrocytes. Lab Science. 1980;7:424-426. (In Russ.)

16. Deryugina A.V., Boyarinov G.A., Simutis I.S. et al. Correction by ozonated erythrocyte mass of erythrocyte metabolic parameters and myocardial structure after acute blood loss. Tsitologiya. 2018;60(2):89-95. DOI: 10.31116/tsitol.2018.02.03.(In Russ.)

17. Lifshitz V.M., Sidelnikova V.I. (2007). Medical Laboratory Tests: reference book. 3rd ed., revis. and enlarg. Moscow: Triada-X. 298 p.

18. Novitsky V.V., Ryazantseva N.V., Stepovaya E.A. et al. Molecular disturbances of erythrocytes membrane during pathology of diff erent genesis are the typical reaction of the organism: contours of the problem. Bulletin of Siberian Medicine. 2006;5(2):62-69. DOI: 10.20538/1682-0363-2006-2-62-69. (In Russ.)

19. Tapbergenov S.O., Sovetov B.S., Tapbergenov A.T., Hann E. Metabolic eff ects of combined integration of adenosine and adenosine monophosphate in hyperadenalememia. Science and Healthcare. 2017;2:92-104. (In Russ.)

20. Arashiki N., Takakuwa Y. Maintenance and regulation of asymmetric phospholipid distribution in human erythrocyte membranes: implications for erythrocyte functions. Curr. Оpin. Нematol. 2017;24(3):167-172 DOI: 10.1097/MOH.0000000000000326.

21. Muravev A.V., Maymistova A.A., Tikhomirova I.A. et al. Role of protein kinases of human red cell membrane in deformability and aggregation changes. Human Physiology. 2012;38(2):94-100. (In Russ.)

22. Kolupaeva E.A., Belyaeva L.M. Modern view of fi sh oil. Meditsinskie novosti. 2013;10:40-42. (In Russ.)

23. Barceló-Coblijn G., Murphy E.J., Othman R. et al. Flaxseed oil and fi sh-oil capsule consumption alters human red blood cell n-3 fatty acid composition: a multipledosing trial comparing 2 sources of n-3 fatty acid. Am. J. Clin. Nutr. 2008;88(3):801-809. DOI: 10.1093/ajcn/88.3.801.

24. Ho M., Maple C., Bancroft A. et al. The benefi cial eff ects of omega-3 and omega-6 essential fatty acid supplementation on red blood cell rheology. Prostaglandins Leukot. Essent. Fatty Acids. 1999;61:13-17. DOI: 10.1054/plef.1999.0066.

25. Iskhakova R.R., Sayfullina F.R. Ozone therapy in ophthalmology. Kazan Medical Journal. 2013;94(4):510-516. (In Russ.)

26. Yamaguchi T., Fukuzaki S. ATP eff ects on response of human erythrocyte membrane to high pressure. Biophys. Рhysicobiol. 2019;16:158-166. DOI: 10.2142/biophysico.16.0_158.

27. Gonzalez-Alonso J., Olsen D. B., Saltin B. Erythrocyte and the regulation of human skeletal muscle blood fl ow and oxygen delivery: role of circulating ATP. Circ. Res. 2002;91(11):1046-1055. DOI: 10.1161/01.RES.0000044939.73286.E2.