Development of methods for standardization of a water-soluble sulfur-containing antioxidant sodium 3-(3'-tret-butyl-4'- hydroxyphenyl)propylthiosulfonate (TS-13)

I n t r o d u c t i o n . Oxidative stress plays a key role in the pathogenesis of various human pathologies, including cardiovascular disease, cancer, neurological disorders, Parkinson’s disease, Alzheimer’s disease, inflammatory diseases, muscular dystrophy, liver diseases, and even human aging. The prevalence of the above diseases is a significant challenge for modern medicine. One of the possible solutions is the development of novel pharmaceutical agents for the prevention and comprehensive treatment of these conditions. Such agents include, in particular, antioxidants. A i m . Development of standardization methods for a sulfur-containing antioxidant sodium 3-(3’-tret-butyl-4’-hydroxyphenyl)propylthiosulfonate (TS-13).

M a t e r i a l s  a n d  m e t h o d s . The object of the study was a substance of the water-soluble antioxidant TS-13. The methods of spectrophotometry, voltammetry, titration and thin-layer chromatography were used.

R e s u l t s . The developed methods for the quantitative determination of TS-13 have been validated in accordance with the General Pharmacopeial Monograph 1.1.0012 “Validation of analytical methods”. The correlation coefficient of each method is not less than 0,997, the relative standard deviation is not more than 2%.

C o n c l u s i o n . The results of this study demonstrate the potential for utilizing the proposed techniques in the quality control of a novel water-soluble antioxidant TS-13.

1. Bardaweel S.K., Gul M., Alzweiri M. et al. Reactive oxygen species: the dual role in physiological and pathological conditions of the human body. Eurasian J. Med. 2018;50(3):193-201. DOI: 10.5152/eurasianjmed.2018.17397.

2. Kandalintseva N.V. (2015). Polyfunctional water-soluble antioxidants of phenolic type. Oxidation, Oxidative Stress, Antioxidants: Reports and Abstracts of the All Russian Conference of Young Scientists and VII School named after N.M. Emanuel. Moscow: RUDN University. P. 81–101. (In Russ.)

3. Menshchikova E.B., Lankin V.Z., Kandalintseva N.V. (2012). Phenolic Antioxidants in Biology and Medicine. Saarbrücken. 488 p. (In Russ.)

4. State Pharmacopoeia of the Russian Federation. 15th ed. URL: https://pharmacopoeia.regmed.ru/pharmacopoeia/izdanie-15 (accessed 09.01.2025) (In Russ.)

5. Belikov V.G. (2021). Pharmaceutical Chemistry: Textbook in 2 Parts. Moscow: MEDpress-Inform. 616 p. (In Russ.)

6. Budnikov G.K., Maistrenko V.N., Vyaselev M.R. (2003). Fundamentals of Modern Electrochemical Analysis. Moscow: Mir; Binom. 592 p. (In Russ.)

7. Ferraz B.R.L., Guimarães T., Profeti D., Profeti L.P.R. Electrooxidation of sulfanilamide and its voltammetric determination in pharmaceutical formulation, urine and human serum on glassy electrode. J. Pharm. Anal. 2018;8(1):55-59. DOI: 10.1016/j.jpha.2017.10.004.

8. Mukundwi V.T., Ivanovskaya E.V., Ligostaev A.V. Development of a method for quantitative determination of the hydrophilic antioxidant TF-7 substance by stripping voltammetry. Journal of Siberian Medical Sciences. 2024;8(2):80-89. DOI: 10.31549/2542-1174- 2024-8-2-80-89. (In Russ.)

9. Belenky L.I., Bzhezovsky V.M., Vlasova N.N. (1988). Chemistry of Organic Sulfur Compounds. General Issues. Moscow: Chemistry. 320 p. (In Russ.)

10. Zheligovskaya N.N., Chernyaev I.I. (1966). Chemistry of Complex Compounds. Moscow: Higher School. 387 p. (In Russ.)

11. Illarionova E.A., Syrovatsky I.P., Mitina A.E. (2022). Thin-layer Chromatography and its use in Qualitative Analysis: Textbook. Irkutsk: IGMU. 53 p. (In Russ.)