Estimation of connexin 43 in myocard by experimental modeling of acute ischemia
Abstract
We induced acute ischemia by ligation of the left coronary artery in the area between left atrial appendage and pulmonary trunk. Animals were sacrificed after 1 hour, 3 hours, 12 and 24 hours. Using immunohistochemical methods to determine the intensity of connexin 43 in cardiomyocytes allows to identify sites of acute ischemic myocardial damage. We estimated the distribution of connexin in the area of intercalated discs and Zbands in the myocardium by experimental modeling of acute ischemia in laboratory male rats of “Wistar” line. Immunohistochemical research of myocardium carried out with the purpose to estimate expression severity of connexin 43 can be recommended for use in cases of morphological examination of dead after sudden cardiac death for diagnostic of myocardial ischemia.
About the Authors
S. V. Savchenko
Novosibirsk State Medical University
Russian Federation
Russian Federation
V. P. Novoselov
Novosibirsk State Medical University
Russian Federation
Russian Federation
R. V. Skrebov
Novosibirsk State Medical University
Russian Federation
Russian Federation
A. S. Morozova
Novosibirsk State Medical University
Russian Federation
Russian Federation
V. A. Gritsinger
Novosibirsk State Medical University
Russian Federation
Russian Federation
T. A. Ageeva
Novosibirsk State Medical University
Russian Federation
Russian Federation
S. V. Aidagulova
Novosibirsk State Medical University
Russian Federation
Russian Federation
K. I. Erschov
Novosibirsk State Medical University
Russian Federation
Russian Federation
E. I. Voronina
Novosibirsk State Medical University
Russian Federation
Russian Federation
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Review
For citations:
Savchenko S.V., Novoselov V.P., Skrebov R.V., Morozova A.S., Gritsinger V.A., Ageeva T.A., Aidagulova S.V., Erschov K.I., Voronina E.I. Estimation of connexin 43 in myocard by experimental modeling of acute ischemia. Journal of Siberian Medical Sciences. 2017;(1):19-23.
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