Development of methods for isolating medicinal substances from blood in framework of implementation of the RF Ministry of Health Order No. 933n
https://doi.org/10.31549/2542-1174-2020-2-30-41
Abstract
The aim of the study was to develop a method for screening blood samples, primarily sample preparation, for the detection of narcotic drugs, psychotropic and medicinal substances in the blood by gas chromatography with mass selective detection (GC-MS) for the implementation of the Ministry of Health of the Russian Federation (MH RF) Order No. 933n dated December 18, 2015. The blood sample preparation procedures for screening testing for narcotic drugs, psychotropic and medicinal substances by GC-MS method were developed and validated in accordance with the requirements of the Order No. 933n of the RF Ministry of Health. It was revealed that an ethanol content of up to 10‰ in the blood does not affect the extraction degree of toxicants and the possibility to interpret the results. The efficacy of the model substances extraction from blood samples was evaluated depending on the isolation method. It was found that the liquid-liquid extraction method is better for isolating compounds with high logP, and the solid-phase extraction method is better for isolating compounds with low logP.
About the Authors
P. V. Morov
Togliatti Narcological Dispensary
Russian Federation
Russian Federation
Consulting Chemist, Clinical Diagnostic Laboratory,
St. Petersburg
O. Yu. Strelova
St. Petersburg State Chemical Pharmaceutical University
Russian Federation
Russian Federation
Cand. Sci. (Chem.), Associate Professor, Head, Department of Pharmaceutical Chemistry,
St. Petersburg
V. N. Kuklin
St. Petersburg State Chemical Pharmaceutical University
Russian Federation
Russian Federation
Dr. Sci. (Pharmaceut.), Professor, Department of Pharmaceutical Chemistry,
St. Petersburg
References
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Review
For citations:
Morov P.V., Strelova O.Yu., Kuklin V.N. Development of methods for isolating medicinal substances from blood in framework of implementation of the RF Ministry of Health Order No. 933n. Journal of Siberian Medical Sciences. 2020;(2):30-41. (In Russ.) https://doi.org/10.31549/2542-1174-2020-2-30-41
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