Microbiota of fetal membranes in intact amniotic sac and full-term pregnancy
https://doi.org/10.31549/2542-1174-2021-1-4-11
Abstract
Aim of research. To study the microbial landscape of intact fetal membranes in full-term pregnancy.
Materials and methods. In 19 pregnant women (mean age — 31.0 ± 5.3 years, mean gestational age — 39.3 ± 0.65 weeks) with intact fetal membranes, the fetal membrane tissue was collected during elective cesarean section to detect by polymerase chain reaction the following microorganisms: Lactobacillus spp., Enterobacteriaceae, Streptococcus spp., Staphylococcus spp., Gardnerella vaginalis/Prevotella bivia/Porphyromonas spp., Eubacterium spp., Sneathia spp./Leptotrihia spp./ Fusobacterium spp., Megasphaera spp./Veillonella spp./ Dialister spp., Lachnobacterium spp./Clostridium spp., Mobiluncus spp./Corynebacterium spp., Peptostreptococcus spp., Atopobium vaginae, Mycoplasma hominis, Ureaplasma (urealyticum + parvum), Candida spp., Mycoplasma genitalium.
Results. Sterile membranes were found in 5 pregnant women (26.3%), in the remaining cases, the total bacterial load (TBL) was 1045 (1035-1058) genome equivalents (GE) per sample. Representatives of the Enterobacteriaceae family prevailed — 1045 GE per sample on average, only in one case Candida spp. were detected. In 42.1% of cases, when determining TBL, specific types of microorganisms were not identified.
Conclusion. On the fetal membranes in full-term pregnancy, the average TBL corresponding to 1045 (1035105 8) GE per sample, in which Enterobacteriaceae prevail in the amount of 104 5 GE per sample on average, is acceptable.
About the Authors
M. A. Kaganova
Samara State Medical University
Russian Federation
Russian Federation
Kaganova Mariya Aleksandrovna — Cand. Sci. (Med.), Assistant Professor, Obstetrics and Gynecology Department.
89, Chapayevskaya str., Samara, 443079
N. V. Spiridonova
Samara State Medical University
Russian Federation
Russian Federation
Spiridonova Natalya Vladimirovna — Dr. Sci. (Med.), Professor, Head, Obstetrics and Gynecology Department.
Samara
E. A. Makhlina
Consultative clinic Panatseya, LLC
Russian Federation
Russian Federation
Makhlina Elena Alekseevna — Obstetrician-gynecologist.
Samara
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Review
For citations:
Kaganova M.A., Spiridonova N.V., Makhlina E.A. Microbiota of fetal membranes in intact amniotic sac and full-term pregnancy. Journal of Siberian Medical Sciences. 2021;(1):4-11. https://doi.org/10.31549/2542-1174-2021-1-4-11
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