The study of the preventive effects of the lipid fraction from the marine green alga Ulva lactuca on the fatty acid composition of phospholipid fractions of erythrocyte membranes
https://doi.org/10.31549/2542-1174-2019-1-98-108
Abstract
The data on the study of the quantitative characteristics of fatty acids in the phospholipid fractions (phosphatidylcholine and phosphatidylethanolamine) of erythrocyte membranes under the action of physical stress (vertical fixation of rats for the dorsal cervical fold) are presented. The appearance of new molecular types of phospholipids due to an increase in saturated fatty acids and a decrease in polyunsaturated fatty acids is shown. With the introduction of a lipid complex from the extract of marine green alga Ulva lactuca and the commercial drug “Essentiale” during the period of stress there was a recovery of the fatty acid spectrum of red blood cell phospholipids. The action of the lipid complex of Ulva lactuca was more effective than Essentiale.
About the Authors
N. F. KushnerovaRussian Federation
Kushnerova Natalya Fedorovna — Dr. Sci. (Biol.), Professor, Head of the Laboratory of Biochemistry, V.I. Il’ichev Pacific Oceanological Institute.
43, Baltiyskaya Str, Vladivostok, 690041.
T. V. Momot
Russian Federation
Momot Tatyana Viktorovna — Cand. Sci. (Med.), Head of the Department of Biochemistry and Biophysics, Far Eastern Federal University, School of Biomedicine.
Vladivostok.
S. E. Fomenko
Russian Federation
Fomenko Svetlana Evgenievna — Cand. Sci. (Biol.), Leading Researcher of the Laboratory of Biochemistry, V.I. Il’ichev Pacific Oceanological Institute.
43, Baltiyskaya Str, Vladivostok, 690041.
V. G. Sprygin
Russian Federation
Sprygin Vladimir Gennadievich — Cand. Sci. (Biol.), Leading Researcher of the Laboratory of Biochemistry, V.I. Il’ichev Pacific Oceanological Institute.
43, Baltiyskaya Str, Vladivostok, 690041.
References
1. Kostyuk V.A., Potapovich A.A. (2004). Bioradicals and Bioantioxidants. Minsk: BSU, 174 p. In Russ.
2. Momot T.V., Kushnerova N.F., Rachmanin Yu.A. (2016). Prevention of deteriorations of blood biochemical indices in experimental stress in rats. Hygiene and Sanitation, 95, 7, 678–681.
3. Kushnerova T.V., Fomenko S.E., Kushnerova N.F. et al. (2010). Antioxidant and membrane-protective properties of an extract from brown alga Laminaria japonica. Rus. J. Marine Biology, 36, 5, 384–389.
4. Fomenko S.E., Kushnerova N.F., Spryigin V.G., Momot T.V. (2013). Disturbances in metabolic processes in liver of rats exposed to stress. Pacific Med. J., 2, 67–70.
5. Imbs T.I., Krasovskaya N.P., Ermakova S.P. et al. (2009). Comparative study of chemical composition and antitumor activity of agueous-ethanol extracts of brown algae Laminaria cichorioides, Costaria costata, and Fucus evanescens. Rus. J. Marine Biology, 35, 2, 164–170.
6. Sprygin V.G., Fomenko S.E., Kushnerova N.F. (2014). Protective effect of the lipid fraction of marine green algae Ulva fenestrate at damage of the liver of rats with carbon tetrachloride. Fundamental researches, 8, 1, 110–114. In Russ.
7. Sanina N.M., Goncharova S.N., Kostetsky E.Y. (2008). Seasonal changes of fatty acid composition and thermotropic behavior of polar lipids from marine macrophytes. Phytochemistry, 69, 1517–1527.
8. Folch J., Less M., Sloane-Stanley G.H. (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226, 1, 497–509.
9. Saratikov A.S., Ratkin A.V., Frolov V.N., Chuchalin V.S. (2004). Influence of hepatoprotectors of phospholipid nature on the toxicity of cyclophosphane. Issues of Biological, Medical and Pharmaceutical Chemistry, 2, 43–47. In Russ.
10. Novgorodtseva T.P., Endakova E.A., Yan’kova V.I. (2003). Manual Methods of “Lipid peroxidation — Antioxidant Protection” System Parameters Research in Biological Fluids. Vladivostok: Far Eastern Federal University, 80 p. In Russ.
11. Svetachev V.I., Vaskovsky V.E. (1972). A simplified technique for thin layer microchromatography of lipids. J. Chromatogr., 67, 2, 376–378.
12. Burchfield H., Storrs E. (1964). Gas Chromatography in Biochemistry (Trans. from English). Moscow: Mir, 500 p. In Russ.
13. Momot T.V., Kushnerova N.F., Rachmanin Yu.A. (2016). Modification of the pattern fatty the acids composition of erythrocytes’ membranes due to the acetone intoxication. Hygiene and Sanitation, 95, 8, 782–785.
14. Kushnerova N.F., Momot T.V., Rakhmanin Yu.A. et al. (2018). The experimental study of the preventive treatment with Viburnum Sargentii K. and Eleutherococcus on the composition of fatty acids of phospholipid components of erythrocyte membranes exposed to intoxication with nitric oxides. Hygiene and Sanitation, 97, 5, 399–404.
15. Fomenko S.E., Kushnerova N.F., Lesnikova L.N. (2012). Experimental asessment of the efficacy of Purple Ascidium Tunic extract for erythrocyte membrane repair upon carbon tetrachloride intoxication. Pharm. Chem. J., 46, 10, 29–34.
Review
For citations:
Kushnerova N.F., Momot T.V., Fomenko S.E., Sprygin V.G. The study of the preventive effects of the lipid fraction from the marine green alga Ulva lactuca on the fatty acid composition of phospholipid fractions of erythrocyte membranes. Journal of Siberian Medical Sciences. 2019;(1):98-108. https://doi.org/10.31549/2542-1174-2019-1-98-108