Evaluation of protein changes of the myocardium in acute ischemia according to the immunohistochemical study
Abstract
Ischemic heart disease is the leading cause of mortality in the developed countries; accordingly the study of various aspects of this disorder is a crucial medico-biological task. Cardiac muscle of the left ventricle taken from 37 individuals suddenly deceased from acute forms of ischemic heart disease (as a result of acute coronary insufficiency and acute myocardial infarction at prenecrotic stage) served as the material for the conducted study. The control group included the cardiac muscles taken from 5 individuals deceased from traumatic brain injury. Besides light and polarized investigation of myocardial sections the immunohistochemical analysis, permitting to evaluate the intensity of cellular protein expression: actin, desmin, connexin 43, was used. The use of immunohistochemical staining of the cardiac muscle allows assessing the status of the macromolecular structure of the myocardium by the expression of actin, desmin and connexin 43 and can be applied for morphological diagnostics of acute focal myocardial injuries in forensic-medical or pathological practice in cases of sudden cardiac death.
About the Authors
S. V. Savchenko
Novosibirsk State Medical University
Russian Federation
Russian Federation
Savchenko Sergey Vladimirovich — Dr. Sci. (Med.), Professor, Head of Forensic Medicine Course of the Forensic Medicine Department.
52, Krasny Prospect, Novosibirsk, 630091
V. P. Novoselov
Novosibirsk State Medical University; Novosibirsk Regional Clinical Bureau of Forensic Medical Examination
Russian Federation
Russian Federation
Novoselov Vladimir Pavlovich — Dr. Sci. (Med.), Professor, Head of the Forensic Medicine Department, NSMU; Head of the NRCBFME
R. V. Skrebov
Novosibirsk State Medical University
Russian Federation
Skrebov Roman Vladimirovich — Post-graduate Student of the Forensic Medicine Department
Russian Federation
Skrebov Roman Vladimirovich — Post-graduate Student of the Forensic Medicine Department
A. S. Grebenshchikova
Novosibirsk State Medical University; Novosibirsk Regional Clinical Bureau of Forensic Medical Examination
Russian Federation
Russian Federation
Grebenshchikova Alina Sergeyevna — Assistant of the Forensic Medicine Department, NSMU; Doctor — Forensic Medical Examiner of Forensic Histological Unit, NRCBFME
V. A. Gritsinger
Novosibirsk State Medical University; Novosibirsk Regional Clinical Bureau of Forensic Medical Examination
Russian Federation
Russian Federation
Gritsinger Valentina Aleksandrovna — Cand. Sci. (Med.), Senior Teacher of the Forensic Medicine Department, NSMU; Doctor — Forensic Medical Examiner of Forensic Histological Department, NRCBFME
T. A. Ageeva
Novosibirsk State Medical University; City Clinical Hospital No. 2
Russian Federation
Russian Federation
Ageyeva Tatyana Avgustovna — Dr. Sci. (Med.), Professor of the Pathologic Anatomy Department, NSMU; Head of the Pathologic Anatomical Department, CCH No. 2
E. I. Voronina
Novosibirsk State Medical University; City Clinical Hospital No. 1
Russian Federation
Russian Federation
Voronina Evgeniya Igorevna — Cand. Sci. (Med.), Assistant of the Pathologic Anatomy Department, NSMU; Mortal Anatomist CCH No. 1
G. M. Kazanskaya
Novosibirsk Medical Research Center named after Academician E.N. Meshalkin
Russian Federation
Russian Federation
Kazanskaya Galina Mikhailovna — Cand. Sci. (Biol.), Senior Researcher of the Pathologic Anatomical Department
E. V. Ovsyanko
Novosibirsk State Medical University
Russian Federation
Russian Federation
Ovsyanko Elena Vladimirovna — Dr. Sci. (Med.), Professor of the Human Anatomy Department
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Review
For citations:
Savchenko S.V., Novoselov V.P., Skrebov R.V., Grebenshchikova A.S., Gritsinger V.A., Ageeva T.A., Voronina E.I., Kazanskaya G.M., Ovsyanko E.V. Evaluation of protein changes of the myocardium in acute ischemia according to the immunohistochemical study. Journal of Siberian Medical Sciences. 2019;(3):95-104.
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