Influence of carbon disulfide intoxication on blood biochemical parameters and their correction by plant phenolic compounds in experiment

Introduction. Phenolic compounds that are part of a variety of phytopreparations have an anti-radical and antioxidant effect.

Aim. Studying the influence of carbon disulfide intoxication on the rat blood biochemical parameters and the possibility of their correction with natural plant phenolic compounds from viburnum.

Materials and methods. The experiment was conducted on white Wistar male rats. Carbon disulfide intoxication (concentration 2.0 mg/m³) was implemented in a special exposure chamber. The animals were divided into 5 groups: the 1st group was control (intact rats); the 2nd group was carbon disulfide intoxication for 3 weeks; the 3rd group was carbon disulfide intoxication for 3 weeks, followed by discontinuation for 7 days; the 4th group was carbon disulfide intoxication for 3 weeks, followed by administration of the viburnum extract for 7 days; the 5th group was carbon disulfide intoxication for 3 weeks, followed by silymarin administration for 7 days. The drugs were administered at a dose of 100 mg of total phenols per kilogram of animal weight.

Results. Carbon disulfide intoxication was accompanied by the development of intense hypercholesterolemia; an increase in the fraction of low-density lipoproteins with simultaneous decrease in high-density lipoproteins in blood serum. There was an increase in the level of malondialdehyde, decrease in the superoxide dismutase activity and reduced glutathione amount, which indicates the depletion of the body’s antioxidant defense system. During the withdrawal period of carbon disulfide intoxication for 7 days, the blood biochemical parameters did not recover, which indicates the preservation of free-radical processes. The administration of the viburnum extract and the comparison drug, silymarin, was accompanied by a recovery to the control group level of blood biochemical parameters after carbon disulfide intoxication.

Conclusion. The toxic effect of carbon disulfide causes a change in the studied blood biochemical parameters. The administration of the viburnum extract and silymarin was accompanied by their restoration to the level of the control group, but a more pronounced effect was observed in the viburnum extract due to the presence of oligomeric forms of polyphenols in its composition.

1. Dashina M.G., Timoshnikova N.N., Novikova Yu.Yu. (2010). Mental disorders in patients with chronic carbon disulfi de intoxication. Health is the Basis of Human Potential: Problems and Ways to Solve, 5, 1, 324–327. In Russ.

2. Liu C.H., Huang C.Y., Huang C.C. (2012). Occupational neurotoxic diseases in Taiwan. Saf. Health Work, 3 (4), 257–267.

3. Wrońska-Nofer T., Chojnowska-Jezierska J., Nofer J.R., Hałatek T., Wiśniewska-Knypl J. (2002). Increased oxidative stress in subjects exposed to carbon disulphide (CS2) — an occupational coronary risk factor. Arch. Toxicol., 76, 152–157.

4. Packer L., Rimbach G., Virgili F. (1999). Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus maritima) bark, pycnogenol. Free Radic. Biol. Med., 27 (5–6), 704–724.

5. Singleton V.L., Orthofer R., Lamuela-Raventos R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth. Enzym., 299, 152–178.

6. Vengerovsky А.I., Markova I.V., Saratikov А.S. (1999). Preclinical study of hepatoprotective agents. Journal of Pharmaceutical Committee, 2, 9–12. In Russ.

7. Sanotsky I.V. (ed.) (1970). Methods for the Determination of Toxicity and Danger of Chemicals (Toxicometry). Moscow, 344 p. In Russ.

8. Paoletti F., Aldinucci D., Mocali A., Caparrini A. (1986). A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Anal. Biochem., 154 (2), 536–541.

9. Novgorodtseva Т.P., Endakova E.А., Yankova V.I. (2003). Guide to Methods for Studying the Parameters of the“Lipid Peroxidation — Antioxidant Protection” System in Biological Fluids. Vladivostok, 80 p. In Russ.

10. Pokrovsky A.A. (ed.) (1969). Biochemical Research Methods in the Clinic. Moscow, 652 p. In Russ.

11. Klingenberg M. (1983). Determination of NAD+ (pp. 253–257). In Bergmеyer H.U. Methods of Enzymatic Analysis. Basel: Verlag Chemie.

12. Kolb V.G., Kamyshnikov V.S. (1982). Clinical Chemistry Handbook. Minsk, 336 p. In Russ.

13. Vaskovsky V.E., Kostetsky E.Y., Vasendin I.M. (1975). A universal reagent for phospholipid analysis. J. Chromatogr., 114 (1), 129–141.

14. Amenta J.S. (1964). A rapid chemical method for quantification of lipids separated by thin-layer chromatography. J. Lipid Res., 5 (2), 270–272.

15. Momot T.V., Kushnerova N.F., Rakhmanin Yu.A. (2016). Prevention of deteriorations of blood biochemical indices in experimental stress in rats. Hygiene and Sanitation, 95, 7, 678–681.

16. Yang T.T., Koo M.W. (2000). Chinese green tea lowers cholesterol level through an increase in fecal lipid excretion. Life Sci., 66 (5), 411–423.