The spectrophotometric determination of A-vitamin activity of carotenoid-containing raw material
https://doi.org/10.31549/2542-1174-2021-4-17-26
Abstract
Carotenoids are of interest as precursors of vitamin A, which has a wide range of pharmacological effects — antioxidant, immunomodulatory, participating in photoreception, etc.
The aim of the research was to develop a technique for the spectrophotometric determination of the A-vitamin activity of carotenoids-rich medicinal plant raw materials (MPRM). The objects of research were the traditional sources of carotenoids — sea buckthorn, carrot, pumpkin and wild rose. A method is proposed for determining the content of individual carotenoids in MPRM by optical density at wavelengths corresponding to the characteristic peaks of α-carotene, β-carotene and lycopene. It has been shown that it is preferable to use isopropyl alcohol as the most effective solvent.
Taking into account the content of specific carotenoids makes it possible to determine the A-vitamin activity of the sum of carotenoids extracted from plant materials, and, accordingly, to increase the efficiency of the use of herbal preparations and biologically active additives for health improvement.
About the Authors
V. V. Velichko
Novosibirsk State Medical University
Russian Federation
Russian Federation
Victoria V. Velichko — Cand. Sci. (Pharmaceut.), Head, Department of Pharmacognosy and Botany
52, Krasny Prospect, Novosibirsk, 630091
D. S. Kruglov
Novosibirsk State Medical University
Russian Federation
Russian Federation
Dmitry S. Kruglov — Cand. Sci. (Tech.), Associate Professor, Department of Pharmacognosy and Botany
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Review
For citations:
Velichko V.V., Kruglov D.S. The spectrophotometric determination of A-vitamin activity of carotenoid-containing raw material. Journal of Siberian Medical Sciences. 2021;(4):17-26. https://doi.org/10.31549/2542-1174-2021-4-17-26
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