Development of a technique for amphotericin B determination by spectrophotometric method in biological fluids
https://doi.org/10.31549/2542-1174-2022-6-2-122-130
Abstract
I n t r o d u c t i o n . The development of methods for determining the concentration of antimicrobial drugs used in various pathological conditions of the body is of great interest.
A i m . Development of a technique for quantitative determination of amphotericin B in blood serum.
M a t e r i a l s a n d m e t h o d s . The drug amphotericin B was used for the preparation of solutions. The optical density of the solutions was determined by the spectrophotometric method.
R e s u l t s . The method of quantitative determination of amphotericin B in sodium chloride solution, which is the closest in composition to biological fl uids, has been tested. Statistical analysis showed that the error of the method for determining
Co n c l u s i o n . A unifi ed method of spectrophotometric determination of amphotericin B in blood serum has been developed. The optimal conditions for the determination of amphotericin in biological fl uids were found: the solvent – 0.9% sodium chloride solution, wavelengths – 363, 383 and 406 nm, t – 3–6°C, detectable concentrations – from 1.0 · 10–2 to 1.0 · 10–5 mg/l.
About the Authors
L. V. Pashkova
Novosibirsk State Medical University
Russian Federation
Russian Federation
Lesya V. Pashkova – Senior Lecturer, Department of Pharmaceutical Chemistry
52, Krasny prosp., Novosibirsk, 630091
E. A. Ivanovskaya
Novosibirsk State Medical University
Russian Federation
Russian Federation
Elena A. Ivanovskaya – Dr. Sci. (Pharmaceut.), Professor, Department of Pharmaceutical Chemistry
Novosibirsk
A. V. Ligostaev
Novosibirsk State Medical University
Russian Federation
Russian Federation
Alexandr V. Ligostaev – Cand. Sci. (Pharmaceut.), Associate Professor, Department of Pharmaceutical Chemistry
Novosibirsk
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Review
For citations:
Pashkova L.V., Ivanovskaya E.A., Ligostaev A.V. Development of a technique for amphotericin B determination by spectrophotometric method in biological fluids. Journal of Siberian Medical Sciences. 2022;(2):122-130. https://doi.org/10.31549/2542-1174-2022-6-2-122-130
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