Evaluation of MIR-143 and MIR-145 genes methylation in lymph nodes of patients with diff use large B-cell lymphoma

I n t r o d u c t i o n . The expression of miR-143 and miR-145 oncosuppressive microRNAs is observed in all types of normal tissues studied and, according to numerous studies, it is lost in malignant neoplasms. A decrease in the expression of these microRNAs in diff use large B-cell lymphoma (DLBCL) is described, the mechanisms of which require additional study. A i m . To evaluate the methylation of MIR-143 and MIR-145 genes in the lymph node tissue of patients with DLBCL and lymph nodes of patients with reactive follicular hyperplasia.

M a t e r i a l s a n d m e t h o d s . The study included biopsies of 14 formalin-fi xed and paraffi nized tumor lymph nodes of patients with DLBCL and 11 lymph nodes of patients with reactive lymphadenopathy. The methylation status of the MIR-145 gene in samples was determined by the method of methyl-specifi c polymerase chain reaction. Direct bisulfi te sequencing based on the Sanger method was used to quantify the methylation of the MIR-143 gene.

R e s u l t s . It was found that all the studied samples, both reactive and tumor, had methylation of the MIR-145 gene. A similar level of methylation of CpG dinucleotides of the MIR-143 gene was registered in all samples of reactive lymph node tissue, whereas tumor samples showed greater heterogeneity. In the samples of DLBCL, namely a non-germinal B cell phenotype, the average level of methylation of the studied fragment of the MIR-143 gene sequence was signifi cantly lower, than in the samples of reactive lymph nodes (p = 0.026).

C o n c l u s i o n . The methylation of MIR-143 and MIR-145 genes detected in the lymph nodes of patients with DLBCL is not tumor-specifi c. The complex cellular composition of the analyzed samples, as well as the diff erent density of microvessels, may explain the diff erences in the level of MIR-143 methylation in the tissue of reactive and tumor lymph nodes.

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