DEXTRAN RAISES RATIO IFN-Γ / IL-10 IN MACROPHAGES OF THE PERSON INFECTED WITH TUBERCULOSIS MYCOBACTERIA
Abstract
The purpose of the research was studying of dextran as ligand of mannose receptor influences on cytokine response of macrophages of the person at infection with micobacteria. Macrophages were incubated with dextran, and then they were infected with H37Rv tuberculosis micobacteria. In 1 and 7 days supracellular liquid was collected and analyzed on concentration of IFN-γ and IL-10. The dextran raised ratio of IFN-γ/IL-10 by 2,4 times. It testifies to shift of activation of macrophages towards a classical way (M1) that promotes formation of Th1 of the immune response that is necessary for a benign outcome at tuberculosis.
About the Author
S. V. Pustylnikov
FBHE «Novosibirsk SRI of tuberculosis» of Ministry of Health
Russian Federation
Russian Federation
References
1. Laminin and fibronectin treatment leads to generation of dendritic cells with superior endocytic capacity / S. Garcia-Nieto [et al.] // PLoS ONE. - 2010. - Vol. 5.
2. The mannose receptor mediates uptake of pathogenic and nonpathogenic mycobacteria and bypasses bactericidal responses in human macrophages / С. Astarie-Dequeker [et al.] // Infection and immunity. - 1999. - Vol. 67.
3. Schlesinger L. S. Macrophage phagocytosis of virulent but not attenuated strains of Mycobacterium tuberculosis is mediated by mannose receptors in addition to complement receptors / L. S. Schlesinger // J. Immunol. - 1993. - Vol. 150, N 7. - Р. 2920-30.
4. The human macrophage mannose receptor directs Mycobacterium tuberculosis lipoarabinomannan-mediated phagosome biogenesis / Р. В. Kang [et al.] // J. Exp. Med. - 2005. - Vol. 202, N 7. - Р. 987-99.
5. Cross-linking of the mannose receptor on monocyte-derived dendritic cells activates an anti-inflammatory immunosuppressive program / М. Chieppa [et al.] // J. Immunol. - 2003. - Vol. 171. - Р. 4552-60.
6. Pulmonary M. tuberculosis infection delays Th1 immunity via immunoadaptor DAP12-regulated IRAK-M and IL-10 expression in antigen-presenting cells / М. Jeyanathan [et al.] // Mucosal immunology. - 2014. - Vol. 7, N 3. - Р. 670-683.
7. Pustylnikov S. Dextrans as Ligands of Mannose Receptor and DC-SIGN Family Receptors - A Novel Means of Modulating Th1/Th2 Immune Responses (Abstract Book) / S. Pustylnikov // Annals of Allergy, Asthma & Immunology. - 2012. - Vol. 109. - C. A108-P248.
8. Targeting the C-type lectins-mediated host-pathogen interactions with dextran / S. Pustylnikov [et al.] // Journal of Pharmacy & Pharmaceutical Sciences. - 2014. - Vol. 17, N 3. - Р. 371-392.
9. Anti-interleukin-10R1 monoclonal antibody in combination with bacillus Calmette-Guerin is protective against bladder cancer metastasis in a murine orthotopic tumour model and demonstrates systemic specific anti-tumour immunity / M. R. Newton [et al.] // Clinical and experimental immunology. - 2014. - Vol. 177, N 1. - Р. 261-268.
10. Interferon-gamma/IL-10 ratio defines the disease severity in pulmonary and extra pulmonary tuberculosis / В. Jamil [et al.] // Tuberculosis. - 2007. - Vol. 87, N 4. - Р. 279-287.
11. Immunoevasion and immunosuppression of the macrophage by Mycobacterium tuberculosis / Z. Hmama [et al.] // Immunological reviews. - 2014. - Vol. 264, N 1. - Р. 220-232.
12. Martinez F. O. The M1 and M2 paradigm of macrophage activation : time for reassessment / F. O. Martinez, S. Gordon // F1000prime reports. - 2014. - Vol. 6.
13. Pulmonary overexpression of IL-10 augments lung fibrosis and Th2 responses induced by silica particles / V. Barbarin [et al.] // American Journal of Physiology-Lung Cellular and Molecular Physiology. - 2005. - Vol. 288, N 5. - Р. L841-L848.
14. Robinson C. M. Interleukin-12 and interleukin-27 regulate macrophage control of Mycobacterium tuberculosis / C. M. Robinson, G. J. Nau // Journal of Infectious Diseases. - 2008. - Vol. 198, N 3. - Р. 359-366.
15. Gene expression profiling of human macrophages at late time of infection with Mycobacterium tuberculosis / E. Volpe [et al.] // Immunology. - 2006. - Vol. 118, N 4. - Р. 449-460.
16. Pustylnikov S. Dextran affects the immune response of BALB/c mice to intranasal challenge with inactivated mycobacterium H37Rv - Abstract in Russian / S. Pustylnikov, E. Zavjalov, E. Litvinova // Russian Laboratory Animal Science Association Conference, 2013, Novosibirsk, Russia, September 25-28. - Novosibirsk, 2013. - Р. 39.
17. Immunogenicity of Danish-SSI 1331 BCG vaccine in the UK : comparison with Glaxo-Evans 1077 BCG vaccine / P. Gorak-Stolinska [et al.] // Vaccine. - 2006. - Vol. 24, N 29. - Р. 5726-5733.
Review
For citations:
Pustylnikov S.V. DEXTRAN RAISES RATIO IFN-Γ / IL-10 IN MACROPHAGES OF THE PERSON INFECTED WITH TUBERCULOSIS MYCOBACTERIA. Journal of Siberian Medical Sciences. 2015;(4):36.
Views:
162
Email this article 