Aim of the research. To assess the long-term results of therapy over 10 years in patients with classical Hodgkin’s lymphoma (cHL) in Novosibirsk.
Materials and Methods. The study included 362 patients with cHL who were followed up in the Hematology Department of the City Clinical Hospital No. 2 in Novosibirsk from January 2008 to December 2018.
Results. Ten-year and 5-year overall survival (OS) in patients with early-stage (I and II) cHL was 95.1 and 98% respectively, in patients with stages III and IV — 89.2 and 90.5% respectively. Relapse-free survival (RFS) of the I and II stage patients was equal to 85%, while in patients with stage III and IV — 66.5% (p = 0.01). The survival rates of patients with cHL with a favorable prognosis within 5 and 10 years after treatment were equal to 100%, while in patients with a poor prognosis these values were 91.7 and 88.9% respectively. Five-year RFS in patients with a favorable prognosis was 95%, ten-year RFS — 91%, in patients with a poor prognosis — 80.8 and 70% respectively (p = 0.001).
Conclusion. The findings indicate a high percentage of survival in patients with cHL and are comparable with the results of other research teams. Thus, the indicators of 5-year overall and relapse-free survival when using combined methods exceed 95 and 80% respectively. Determination of the prospects for improving treatment results dictates the need to continue further scientific search for optimal risk-adapted cHL treatment regimens.

The purpose of the study is to determine the pharmacokinetic parameters of a new ester derivative of indomethacin — codenamed indomenthyl — after its administration to rats. Indomenthyl was administered into stomach of rats as a solution in flaxseed oil: single administration at a dose of 12.5, 25 and 50 mg/kg, or multiple administration once a day at a dose of 50 mg/kg for 7 days. Blood plasma levels of indomenthyl and its active metabolite indomethacin were detected using a high-performance liquid chromatograph with the ultraviolet spectrophotometric detector.
It was shown that the value of the systemic exposure AUC of indomenthyl and indomethacin, released during its hydrolysis, increases linearly in the dose range of 12.5–50 mg/kg. On repeated (7-fold) administration indomenthyl and indomethacin do not cumulate in the organism. Thus, indomenthyl may be considered a safe retarded release form of indomethacin for the treatment of inflammatory diseases.

Experiments on rats have shown that ethanol at a dose of 4.5 g/kg leads to an increase in the activity of lactate dehydrogenase (LDH) in rat liver cells in a direct reaction in subcellular fractions. In the cytoplasmic fraction, the LDH activity in the reverse reaction decreased. Pre-administration of heparin to animals at a dose of 250 IU/kg partially neutralizes the effects of intraperitoneal administration of ethanol, which is expressed in a decrease in the activity of LDH in rat liver cells in a direct reaction in subcellular fractions. Binding of endogenous heparin to protamine sulfate and subsequent administration of ethanol leads to an increase in LDH activity in the mitochondrial fraction of rat liver cells.

Acute psychodysleptic poisoning is accompanied with the activation of anaerobic processes, development of hypoxia, rhabdomyolysis and an inflammatory reaction, which ultimately determines the occurrence of complications (cerebral ischemia, hypotension, hepatorenal syndrome, pneumonia, etc.). In order to improve the intensive care of patients with acute psychodysleptic poisoning, a study was carried out in which 148 patients with acute psychodysleptic poisoning participated. The control group consisted of 75 patients who received conventional therapy in accordance with Federal Clinical Guidelines. The study group included 73 patients who were additionally prescribed ethylmethylhydroxypyridine succinate (mexidol).

Acute psychodysleptic poisoning, even mild, is accompanied by the development of metabolic disorders that cannot be corrected by standard therapy, despite the consciousness recovery and relief of tachycardia. Acute psychodysleptic poisoning of moderate severity is characterized by the development of anaerobic processes, hepatorenal syndrome and inflammatory response. Conventional therapy could not stop the pathological reactions in a short time. Additional use of mexidol contributed to a decrease in the intensity of anaerobic processes and the elimination of metabolic disorders, which made it possible to shorten the rehabilitation period by 3–4 times.

In order to study the etiological factors and pathomorphological changes in lungs in congenital pneumonia, 80 case histories and autopsy records of deceased children who were admitted to the pathological departments of multidisciplinary children’s hospitals in Novosibirsk in 2018–2019 were analyzed. Such parameters as gender, age, maturity, data from intravital and postmortem bacteriological tests, as well as the results of histological examination of internal organs and, taking into account the continuity of the mother — placenta — fetus system and the role of the liver as a barrier organ in perinatal infections, placenta and liver, were assessed.
It was found that fatal outcomes due to congenital pneumonia are more often observed in boys, and mainly in premature newborn infants. Congenital pneumonia in most cases was part of a generalized infectious process (neonatal sepsis, intrauterine infection) (up to 85%), with frequent damage to the intestine (enterocolitis), liver (fetal hepatitis). The main etiological factors of congenital pneumonia are Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli or bacterial-bacterial associations of the above pathogens. The morphological picture of the lungs was dominated by desquamative interstitial pneumonia.

A comparative analysis of the dynamics of autonomic regulation in the process of training and recovery period in athletes of games-based (volleyball and tennis — 38 individuals) and strength (jiu-jitsu and karate — 42 individuals) sports was carried out. The age of the study participants was 18–25 years. It was found that with similar changes for both groups in the regulation of the heart rate the decrease in the central nervous system influence on cardiac rate in games-based sportsmen is less prominent. Restoration of the regulatory influences on the heart is provided by changing the factor that was the main one in ensuring the heart rhythm during training: in games-based sports athletes due to parasympathetic mechanisms activation; in strength sports athletes — by restoring suprasegmental influences implemented through sympathetic centers.

Aim. Evaluation of quality of life of coronary heart disease (CHD) patients with normal and lowered left ventricular ejection fraction (LVEF) treated by endomyocardial implantation of autologous bone marrow mononuclear cells (ABMMCs) by using the NOGA cardiac navigation system in combination with modern drug therapy.
Materials and methods. CHD patients (n=48) were divided into two groups according to their initial LVEF: group I patients had LVEF ≥ 50% (n = 30), while in group II it was 36–49% (n = 18). All patients underwent endomyocardial implantation of ABMMCs combined with modern drug therapy of CHD. Bone marrow aspiration to obtain ABMMC was performed from the iliac crest under regional anesthesia on the day of cell implantation according to a standard technique. Another option for obtaining ABMMCs was isolation from peripheral blood after administration of recombinant human granulocyte colony-stimulating factor (GCSF) (Grasalva, Israel). Each ischemic segment of the myocardium got 10 injections of cells of 0.2 ml volume. The average number of autologous cells injected into every patient was 41 ± 16 × 10⁶. The fraction of CD34/CD45-positive cells amounted to 2.5 ± 1.6%. Quality of life was evaluated using the SF-36 Health Survey at all stages of control (baseline, 6 months, 1 year and 3 years).
Results. No intraoperative complications were observed in both groups. The patients were discharged from the hospital on 3^rd–5^th day. At all stages of control all studied indicators of quality of life demonstrated a statistically significant improvement in relation to the baseline data in patients of both groups. As for complete functional relationship (W), the most significant parameters were as follows: PF (Physical Functioning) (W = 0.54), RE (Role-Emotional) (W = 0.72), SF (Social Functioning) (W = 0.48).
Conclusion. Endomyocardial implantation of ABMMCs supported by modern drug therapy facilitates the parameters of quality of life improvement in CHD patients with normal and moderately lowered LVEF during 3-year follow-up irrespective of its baseline value.

Aim . To investigate structural changes developing in gingival fibroblasts of marginal chronic gingivitis (CG) patients after comprehensive therapy including anode galvanic stimulation of the gums.
Materials and methods . A study was conducted using the methods of light and electron microscopy, morphometry of gingival tissue samples of 39 individuals (10 patients with intact gums, 10 patients with CG before treatment, and 19 patients with CG after treatment including, in addition to professional removal of dental calculus, training in oral hygiene techniques, monitoring the level of oral hygiene and 3-fold gingival anode galvanic stimulation for 5 min which allows to stop swelling and bleeding of gums as soon as possible).
Results . In the gingival fibroblasts of patients with chronic gingivitis after treatment, which included anode-galvanization, structural signs of a decrease in the synthetic activity of cells were detected. An increase in the volume density of collagen fibers was found in the extracellular matrix of the gingival connective tissue layer, probably due to the dehydration of the gum tissue under effect of direct electric current.
Conclusion . The identified structural changes may affect the repair processes in the periodontium.

Hemolytic uremic syndrome (HUS) is a special form of thrombotic microangiopathy (TMA) characterized by obligate kidney damage. The development of HUS is most often associated with enterohemorrhagic escherichiosis and shigellosis, however, at the present stage there are reports of new bacterial pathogens that may be responsible for the development of this form of TMA. One of them is Campylobacter. The HUS pathogenesis in infections caused by Campylobacter is unknown, which does not allow us to develop adequate approaches to the diagnosis, treatment and rehabilitation of these conditions. In 2018 there were recorded two cases of HUS in children in Novosibirsk, Campylobacter was identified as the etiological agent. These cases are described in the article. The presented description demonstrates the complexity of patient management and the high urgency of Campylobacter-associated HUS. The analysis makes the Shiga-toxin mediated mechanism of TMA development in campylobacter infections doubtful, however, it does not allow to exclude both the neuraminidase and autoimmune genesis of such changes.
Thus, Campylobacter-associated HUS requires further study in order to develop and optimize approaches to the diagnosis and treatment of this condition.

Attempts to optimize the diagnosis of sepsis and its complications have led to the development of Sepsis-3 concept which contradicts the basic provisions of therapy for this threatening condition, namely, the earliest possible onset of the treatment. In turn, the introduction of new methods for monitoring and substitution of organ functions in multiple organ failure did not cause a noticeable improvement in the results of sepsis therapy. The high incidence of antibiotic-resistant strains also requires the search of the new approaches in the diagnosis and therapy of sepsis.
The present review is intended to focus on such a typical sepsis phenomenon as absolute lymphopenia. Numerous studies show that it is lymphopenia that determines the severity of sepsis. Composite biomarkers (the neutrophil-lymphocyte count ratio, NLCR) or lymphocyte index (the lymphocyte/peripheral blood granulocytes ratio)) are the most reliable criteria in sepsis diagnosis. In addition, the presented data suggest that lymphopenia correction significantly improves prognosis in sepsis. It seems clear that the acknowledgment of the absolute lymphopenia’s key role in pathogenesis, diagnosis and therapy of sepsis will serve as an impulse for further development of sepsis concept.

The literature review considers connective tissue dysplasias and their role in the pathogenesis of diseases of the cardiovascular, respiratory, immune and other systems of the body. The etiological and morphological aspects of this pathology, classification, as well as clinical features of the course of some diseases associated with connective tissue dysplasia in both adults and children are presented.